We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Evaluation of eotaxin-1, -2, and -3 protein production and messenger RNA expression in patients with vernal keratoconjunctivitis.
- Authors
Noriko Inada; Mitsuru Sawa
- Abstract
Abstract Purpose To evaluate the in vivo expression of members of the eotaxin subfamily, eotaxin-1, -2, and -3, at the ocular surface, we analyzed the messenger RNA (mRNA) expression of the eotaxin subfamily in conjunctival epithelium and the protein expression of the eotaxin subfamily in tears of patients with vernal keratoconjunctivitis (VKC) and in those of healthy individuals. Methods The subjects were 25 patients with VKC (25 eyes) and 11 healthy volunteers (11 eyes) as a control. Tear samples were collected using the Schirmer strip method. Tear samples were eluted, and concentrations of eotaxin-1, -2, and -3 in the tear samples were determined by enzyme-linked immunosorbent assay (ELISA). Concentration of eosinophil cationic protein (ECP) in tears was also determined by chemiluminescent enzyme immunoassay. Conjunctival epithelial cells were obtained from upper tarsal conjunctiva by impression cytology, and eotaxin-1, -2, and -3 mRNA extracted from the impression cytology membrane were analyzed by reverse transcriptase polymerase chain reaction (RT-PCR). Conjunctival smears, which were obtained by tarsal conjunctival scraping, were stained for eotaxin-2 using immunohistochemical methods. Results In the ELISA analysis, the expression ratio of eotaxin-1 (P P r = 0.53, P P P P Conclusions We showed that the mRNA expression and the protein production of the eotaxin subfamily at the ocular surface are critical biomarkers when investigating the pathophysiology of eosinophilic inflammation and the effect of antiallergic treatment in patients with VKC.
- Subjects
GENE expression; MESSENGER RNA; KERATOCONJUNCTIVITIS; EYE diseases; CONJUNCTIVA; EPITHELIAL cells; TEARS (Body fluid); BODY fluid analysis; REVERSE transcriptase polymerase chain reaction; PATIENTS
- Publication
Japanese Journal of Ophthalmology, 2009, Vol 53, Issue 2, p92
- ISSN
0021-5155
- Publication type
Article