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- Title
食品中の6種甘味料分析における透析膜に試料を充填しない 迅速透析法.
- Authors
田原正一; 山本純代; 石井悦子; 小林千種
- Abstract
A simple and rapid dialysis method was developed for the analysis of sweeteners such as aspartame, sucralose,dulcin, acesulfame potassium,sodium saccharin, and sodium cyclamate present in foods. In the conventional method, a suspension was prepared by mixing 20 g of food with the dialysate and added to the dialysis membrane; the final volume was made up to 200 mL with the addition of the dialysate. The dialysis continued for 24-48 hours, after which the sweeteners that permeated the dialysis membrane were analyzed by high performance liquid chromatography. In the development method, a suspension was prepared by mixing 5 g of food with the dialysate and a dialysis membrane containing 2 mL of the dialysate was added; the final volume made up 50 mL with the addition of the dialysate. The dialysate comprised of 30% methanol containing 0.5% ammonium formate. After 90 minutes, the inner solution of the dialysis membrane was taken and analyzed by the liquid chromatograph-photodiode array-mass spectrometry. Using the above protocol, a recovery rate from 88.8 to 109.4% and a % RSD from 0.2 to 4.8 was obtained.
- Subjects
DIALYSIS (Chemistry); ASPARTAME; SUCRALOSE; LIQUID chromatography; MASS spectrometry
- Publication
Japanese Journal of Food Chemistry & Safety, 2020, Vol 27, Issue 2, p76
- ISSN
1341-2094
- Publication type
Article