We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Preparation of an allergen-specific immunomodulator by phosphorylation of a major buckwheat globulin allergen, Fag e 1, with diminished IgE response via Tfh cell activation.
- Authors
Al Athamneh, Ahmad M.; Supatta Chawalitpong; Yuta Suzuki; Daiki Yamaguchi; Soichiro Nakamura; Shigeru Katayama
- Abstract
Fag e 1 is a 22 kDa globulin found in common buckwheat (Fagopyrum esculentum) and it is known to be one of the major allergens causing severe allergic symptoms. In this study, we successfully obtained recombinant Fag e 1 using the Pichia expression system and prepared an allergen-specific hypoallergenic agent by the controlled dry-heating phosphorylation of Fag e 1 (P-Fag e 1). Then, we investigated if P-Fag e 1 can be useful as an immunomodulator in Fag e 1-sensitized mice. For this, P-Fag e 1 was orally administrated into Fag e 1-sensitized mice for 6 weeks, and then these mice were challenged with Fag e 1. We observed a significant reduction in the histamine release in addition to diminished production of total as well as specific IgE in the P-Fag e 1-treated mice. In contrast, total IgA level increased by the treatment with P-Fag e1. The levels of the IL-4 cytokines from both spleen and Peyer's patches were significantly decreased in P-Fag e 1 treated mice. Additionally, the population of T follicular helper cells (Tfh cells) was increased in the P-Fag e 1 treated group. The suppression of IgE production in the Fag e1 treated group might be due to the enrichment of the Tfh cells and IgA production. Therefore, it could be proposed that P-Fag e 1 is an allergen-specific immunomodulator in mice allergic to Fag e 1.
- Subjects
ALLERGENS; IMMUNOMODULATORS; PHOSPHORYLATION; BUCKWHEAT; RAT physiology; T cells
- Publication
Japanese Journal of Food Chemistry & Safety, 2019, Vol 26, Issue 2, p91
- ISSN
1341-2094
- Publication type
Article