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- Title
Guanine Exchange Factor P92GEF target RhoA and Inhibit Cell Proliferation and Invasion as a Tumor gene.
- Authors
TANG Qiu-lin; LANG Nan; LI Li-bo; SHI Tang; BI Teng
- Abstract
Dbl family guanine exchange factor (GEFs) is the main regulatory unit for the malignant transformation of Rho family proteins. It plays an important role in the transformation of Rho protein from the inactive GDP form to the active GTP form and participates in the cytoskeleton rearrangement, the cell growth and vitality. This study conducted a preliminary structural and functional study of a typical family member, P92GEF, and discussed the role of the molecule in the development of tumor. The expression of P92GEF in 48 normal tissues of human body was measured by Realtime PCR; GST-pulldown technique was used to detect the GEF activity of P92GEF in vivo; the dual-luciferase reporter gene detection technique was used to test the transcription factor activity of the small downstream molecules, and the high expression of P92GEF was tested by the double staining immunofluorescence method. Effects of high expression of P92GEF on cell proliferation, invasion and tumorigenic ability in vitro were examined using CCK8, Transwell and soft agar clony formation assays. The results of bioinformatics analysis showed that P92GEF contains 841 amino acids, has typical Dbl family molecular domain. The expression of P92GEF in the lung tissue was highest. P92GEF binds to RhoA in vitro and promotes the proliferation and invasion of NIH3T3 cells, and has significant ability to clone in vitro. P92GEF is a typical guanine exchange factor family molecule, which can activate Rho family RhoA and has obvious oncogene characteristics.
- Subjects
CELL proliferation; GUANINE; GUANINE nucleotide exchange factors; REPORTER genes
- Publication
Progress in Modern Biomedicine, 2019, Vol 19, Issue 16, p3018
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2019.16.004