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- Title
癌胚抗原蛋白芯片的研发及其在肝癌检测中的评价.
- Authors
李康; 张爱英; 张贺; 赵元顺; 张永宏; 李宁
- Abstract
Objective: To develop a simple, rapid, highly specific, and low-cost protein microarray for detecting carcinoembryonic antigen (CEA) levels in serum, and to evaluate it through diagnosis of of hepatocellular carcinoma (HCC). Methods: Using double-antibody sandwich method, the protein microarray were prepared with "sandwich" structure of capture antibody-antigen-detection antibody to detect CEA levels in serum. In the blind experiment, 50 CEA positive serum samples from patients with HCC and 56 serum samples from healthy human were detected by the CEA protein microarray. Results: CEA> 5 ng/m L was as positive criteria, the sensitivity of CEA protein chip was 92 % (46/50), and its specificity was 100 % (56/56). Receiver operating characteristic curve (ROC)analysis showed that area under the ROC curve (AUC) of serum CEA detection by the protein microarray was 0.960, and comparing with the 0.5 the result was statistically significant (P <0.001), and its diagnostic accuracy for CEA was high. Conclusions: The protein microarray for detecting CEA concentration in serum was successfully established, and provided one candidate biomarker to develop multiple biomarkers protein chips for combination detection of HCC.
- Publication
Progress in Modern Biomedicine, 2015, Vol 15, Issue 30, p5806
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2015.30.002