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- Title
Seed analysis of Dichromanthus aurantiacus, terrestrial orchid from Toluca valley, México.
- Authors
Sandoval Reyes, J. B.; Aguilar Morales, M. A.; De la Cruz Olvera, Armandina; Laguna Cerda, Antonio; Cruz Monterrosa, Rosy G.; Diaz Ramírez, Mayra; Jiménez Guzmán, Judith; García Garibay, Mariano; Martínez García, Carlos G.; Rayas Amor, Adolfo A.
- Abstract
Objective The objective of this work was to analyze the viability and germination of Dichromanthus aurantiacus seeds, a terrestrial orchid from Toluca valley, México. Design/methodology/approach The size and color were evaluated. Two methods determined the viability 1) the tetrazolium test (imbibition for 24 hours in the water, 2 hours in calcium hypochlorite (CaCOCl2), and drops of Tween-80). 2) the asymbiotic seed germination by in vitro culture (imbibition for 24 hours in the water, and the concentration of MS medium plus natural extracts). Results The seeds of this specie showed approximately 0.2 mm long and 0.05 mm wide, they possess an embryo and a brownish testa. There were significant differences (P<0.05) between the treatments finding a positive effect with the tetrazolium test, achieving up to 91.4% viability. In the in vitro germination, the imbibition of the seeds favored contamination. The concentration of MS and the addition of natural extract presented significant differences (P<0.05), the 50% MS plus 10% of coconut water showed up to 92.8% of germination at 60 days. Study limitations/implications The results are preliminary of a long-term experiment. Findings/Conclusions The seeds of Dichromanthus aurantiacus showed brown testa and an oval embryo with dimensions of 0.2 mm long and 0.05 mm wide. The tetrazolium test's viability showed 91.4% viability when they were soaked in sodium hypochlorite solution (CaCOCl2) for two hours, 24 hours soaking in tetrazolium solution (1%) plus two drops of Tween-80. The asymbiotic in vitro culture showed up to 92.8% germination in 60 days using MS medium at 50% enriched with 10% coconut water.
- Subjects
MEXICO City (Mexico); GERMINATION; COCONUT water; SEEDS; SODIUM hypochlorite; VALLEYS; ORCHIDS; PHALAENOPSIS
- Publication
Agro Productividad, 2020, Vol 13, Issue 10, p113
- ISSN
2448-7546
- Publication type
Article
- DOI
10.32854/agrop.v13i11.1873