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- Title
Effect of Bradykinin on Cultured Bovine Corneal Endothelial Cells.
- Authors
Yang, Suk-Woo; Lee, Woon Kyu; Lee, Eun-Jung; Kim, Kyoung-Ah; Lim, Young; Lee, Kweon-Haeng; Rha, Hyoung Kyun; Hahn, Tae-Won
- Abstract
Purpose: To clarify the effect of bradykinin on cytosolic free calcium mobilization and cell proliferation in cultured bovine corneal endothelial cells (BCEC). Methods: The cytosolic free calcium concentration (Ca2+]i) was measured with the InCaTM Imaging System after the treatment of bradykinin (10-11 to 10-7 M) alone or with the pretreatments of EGTA, bradykinin receptor (Bk1 and Bk2) antagonists and an inhibition of phospholipase C (U-73122). Also, the effect of bradykinin on cell proliferation in BCEC was evaluated using cell counts. Results: In BCEC, [Ca2+]i in the resting state was 87 ± 9 nM. Bradykinin induced an increment of [Ca2+]i in a concentration-dependent manner and its 50% effective concentration was approximately 5 × 10-11 M. A [Ca2+]i increment at 10-8 M bradykinin was inhibited with the pretreatment of EGTA, an extracellular calcium chelator. U-73122 (5 × 10-6 M) attenuated the bradykinin-induced [Ca2+]i increment. The pretreatment of HOE-140 (Bk2 antagonist) almost attenuated the bradykinin (10-8 M)-induced [Ca2+]i increase, but des-Arg9-[Leu8]-bradykinin (Bk1 antagonist) did not suppress it. To investigate the physiological effect of bradykinin, the effect of bradykinin on cell proliferation was studied. 10-8 M of bradykinin produced a significant increase in cell numbers. This mitogenic effect of bradykinin was inhibited by the Bk2 antagonist. Conclusions: Bradykinin-induced stimulation of the signal transduction pathway in BCEC is coupled with the Bk2 type receptor. Furthermore, bradykinin produces the mitogenic effect in BCEC. Copyright © 2001 S. Karger AG, Basel
- Publication
Ophthalmologica, 2001, Vol 215, Issue 4, p303
- ISSN
0030-3755
- Publication type
Article
- DOI
10.1159/000050879