We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
An Untargeted Metabolomic Analysis of Lacticaseibacillus (L.) rhamnosus , Lactobacillus (L.) acidophilus , Lactiplantibacillus (L.) plantarum and Limosilactobacillus (L.) reuteri Reveals an Upregulated Production of Inosine from L. rhamnosus.
- Authors
Spaggiari, Luca; Pedretti, Natalia; Ricchi, Francesco; Pinetti, Diego; Campisciano, Giuseppina; De Seta, Francesco; Comar, Manola; Kenno, Samyr; Ardizzoni, Andrea; Pericolini, Eva
- Abstract
Lactic acid bacteria are considered an inexhaustible source of bioactive compounds; indeed, products from their metabolism are known to have immunomodulatory and anti-inflammatory activity. Recently, we demonstrated that Cell-Free Supernatants (CFS) obtained from Lactobacillus (L.) acidophilus, Lactiplantibacillus (L.) plantarum, Lacticaseibacillus (L.) rhamnosus, and Limosilactobacillus (L.) reuteri can impair Candida pathogenic potential in an in vitro model of epithelial vaginal infection. This effect could be ascribed to a direct effect of living lactic acid bacteria on Candida virulence and to the production of metabolites that are able to impair fungal virulence. In the present work, stemming from these data, we deepened our knowledge of CFS from these four lactic acid bacteria by performing a metabolomic analysis to better characterize their composition. By using an untargeted metabolomic approach, we detected consistent differences in the metabolites produced by these four different lactic acid bacteria. Interestingly, L. rhamnosus and L. acidophilus showed the most peculiar metabolic profiles. Specifically, after a hierarchical clustering analysis, L. rhamnosus and L. acidophilus showed specific areas of significantly overexpressed metabolites that strongly differed from the same areas in other lactic acid bacteria. From the overexpressed compounds in these areas, inosine from L. rhamnosus returned with the best identification profile. This molecule has been described as having antioxidant, anti-inflammatory, anti-infective, and neuroprotective properties. The biological significance of its overproduction by L. rhamnosus might be important in its probiotic and/or postbiotic activity.
- Subjects
LACTOBACILLUS rhamnosus; LACTIC acid bacteria; METABOLOMICS; INOSINE; LACTOBACILLUS; FUNGAL virulence
- Publication
Microorganisms, 2024, Vol 12, Issue 4, p662
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms12040662