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- Title
The Application of Recombinant Phototoxins 4D5scFv-miniSOG and DARPin-miniSOG to Study the HER2 Receptor Internalization.
- Authors
Kuzichkina, E. O.; Shilova, O. N.; Deyev, S. M.; Petrov, R. V.
- Abstract
It was found that the fluorescent properties of the phototoxic domain of miniSOG allow to assess the ability of toxins to bind to human breast adenocarcinoma cells SK-BR-3 and study the dynamics of their internalization. We established that the main cause of the decrease of the fluorescence intensity of the recombinant proteins 4D5scFv-miniSOG and DARPin-miniSOG during their internalization in the complex with the HER2 receptor is their shielding and absorption of the fluorescence of miniSOG by the cells fluorophores.
- Subjects
FLUORESCENCE; TOXINS; ADENOCARCINOMA; CANCER cells; RECOMBINANT proteins; FLUOROPHORES
- Publication
Doklady Biochemistry & Biophysics, 2018, Vol 482, Issue 1, p245
- ISSN
1607-6729
- Publication type
Article
- DOI
10.1134/S1607672918050046