We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effect of icariin surface treatment on the resorption of denuded roots after replantation in rat.
- Authors
Elhakim, Ahmed; Kim, Sunil; Shin, Su‐Jung; Jung, Han‐Sung; Kim, Euiseong
- Abstract
Aim: Limiting the incidence of resorption associated with delayed replantation of avulsed teeth is critical for long‐term tooth survival. In this study, we assessed whether icariin, a natural product with anti‐osteoclastic properties, could reduce root resorption in a rat model of tooth replantation. Methodology: Cytocompatibility of icariin (10, 20, 40 and 80 μM) was evaluated by CCK‐8 proliferation assay in vitro, and an osteoclastogenesis assay was performed to evaluate the effect of icariin on the differentiation of rat bone marrow macrophages and human peripheral blood monocytes into tartrate‐resistant acid phosphatase‐stained (TRAP+) multinucleated giant cells (MNGCs). Differentiation of human periodontal ligament stem cells (hPDLSCs) treated with icariin (10 μM) was also evaluated at 5, 10 and 21 days of osteogenic induction. The first maxillary molars of five‐week‐old male Sprague–Dawley rats were extracted, denuded of PDL, then treated either with neutralized collagen solution (Carrier control) or icariin in collagen (3 μg/μL) before replantation into their sockets. The animals were euthanized 2 weeks post‐surgery for micro‐computed tomography (micro‐CT) imaging and histological analyses. Results: Icariin was cytocompatible and significantly reduced the differentiation of TRAP+ MNGCs in a dose‐dependent manner compared to the control. Moreover, icariin enhanced alkaline phosphatase activity, expression of osteogenic marker genes and proteins, and calcium deposition in hPDLSCs. Micro‐CT imaging of the replanted samples demonstrated a significantly higher volume of remaining roots in the icariin‐treated group than in the control group. Histological analysis revealed a marked number of resorptive lacunae with TRAP activity in the control group, whereas icariin‐treated samples showed signs of functional healing and reduced osteoclastic activity. Conclusions: Icariin was biocompatible and demonstrated potent anti‐osteoclastic and pro‐osteogenic properties that reduced resorption and promoted functional healing of denuded roots in a rat maxillary first molar model of replantation. These findings indicate that root surface treatment with icariin may be a clinically relevant and practical method for improving the retention and survival of teeth with compromised PDL after delayed replantation following traumatic avulsion.
- Subjects
TOOTH replantation; ROOT resorption (Teeth); SURFACE preparation; MULTINUCLEATED giant cells; OSTEOINDUCTION; X-ray computed microtomography; RATS
- Publication
International Endodontic Journal, 2023, Vol 56, Issue 12, p1550
- ISSN
0143-2885
- Publication type
Article
- DOI
10.1111/iej.13980