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- Title
Evaluation of safety and immunogenicity of duck-plague virus gC/gE double gene deletion.
- Authors
Peilin Ruan; Xin Feng; Anchun Cheng; Mingshu Wang; Wei Zhang; Ying Wu; Qiao Yang; Bin Tian; Xuming Ou; Di Sun; Shaqiu Zhang; Sai Mao; Dekang Zhu; Renyong Jia; Shun Chen; Mafeng Liu; Xin-Xin Zhao; Juan Huang; Qun Gao; Yanling Yu
- Abstract
Duck plague caused by duck plague virus (DPV) is a highly contagious disease that can cause serious morbidity and death in waterfowl such as ducks and geese, and bring huge economic losses to the duck industry. In this study, on the basis of the duck plague virus gC gene deletion strain CHv-DgC, based on the duck plague virus bacterial artificial chromosome (BAC) platform in our laboratory, the gE gene was knocked out using the traceless deletion technology to obtain gC/gE double gene deletion candidate vaccine strain CHv-DgC/gE. The double gene deletion strain (CHv-DgC/gE) constructed in this study has greatly weakened virulence, no pathogenicity to ducks, and stable genetic characteristics in vitro and in vivo. Ducks immunized with CHv-DgC/gE can produce neutralizing antibodies and ELISA antibody levels comparable to those of commercial duck plague attenuated vaccine immunization, and can resist 100 LD50 CHv challenge of ducks, with good immune protection effect. It has the potential to be further developed into duck plague gC/gE double gene deletion, marked attenuated vaccine.
- Subjects
BACTERIAL artificial chromosomes; IMMUNE response; ACTINOBACILLUS pleuropneumoniae; COMMUNICABLE diseases; BACTERIOPHAGES; CRAYFISH
- Publication
Frontiers in Immunology, 2022, Vol 13, p1
- ISSN
1664-3224
- Publication type
Article
- DOI
10.3389/fimmu.2022.963009