We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
MiR-155 induction by microbes/microbial ligands requires NF-κB-dependent de novo protein synthesis.
- Authors
Cremer, Thomas J.; Fatehchand, Kavin; Shah, Prexy; Gillette, Devyn; Patel, Hemal; Marsh, Rachel L.; Besecker, Beth Y.; Rajaram, Murugesan V. S.; Cormet-Boyaka, Estelle; Kanneganti, Thirumala-Devi; Schlesinger, Larry S.; Butchar, Jonathan P.; Tridandapani, Susheela
- Abstract
MiR-155 regulates numerous aspects of innate and adaptive immune function. This miR is induced in response to Toll-like receptor ligands, cytokines, and microbial infection. We have previously shown that miR-155 is induced in monocytes/macrophages infected with Francisella tularensis and suppresses expression of the inositol phosphatase SHIP to enhance activation of the PI3K/Akt pathway, which in turn promotes favorable responses for the host. Here we examined how miR-155 expression is regulated during infection. First, our data demonstrate that miR-155 can be induced through soluble factors of bacterial origin and not the host. Second, miR-155 induction is not a direct effect of infection and it requires NF-κB signaling to up-regulate fos/jun transcription factors. Finally, we demonstrate that the requirement for NF-κB-dependent de novo protein synthesis is globally shared by microbial ligands and live bacteria. This study provides new insight into the complex regulation of miR-155 during microbial infection.
- Subjects
PROTEIN synthesis; MONOCYTES; MACROPHAGES; LIGANDS (Biochemistry); INFECTION; IMMUNITY; MICROBIOLOGY
- Publication
Frontiers in Cellular & Infection Microbiology, 2012, Vol 2, p1
- ISSN
2235-2988
- Publication type
Article
- DOI
10.3389/fcimb.2012.00073