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- Title
Effects of salmon DNA fraction in vitro and in a monosodium iodoacetate-induced osteoarthritis rat model.
- Authors
Ho Jong Ra; Mi Young Oh; Hee Ju Kim; Seung Yong Lee; Dae Woon Eom; Suk Kyu Lee; Su-Nam Kim; Kyu Sung Chung; Hyuk Jai Jang
- Abstract
PRF001 is a fragmented DNA polymer extracted from the testes of salmon. The purpose of this study was to assess the anti-inflammatory effect of PRF001 in vitro as well as the protective effect of PRF001 intake against arthritis in a rat model. In vitro, cell survival and inflammatory markers after H2O2 treatment to induce cell damage were investigated in CHON-001 cells treated with different concentrations of PRF001. In vivo, osteoarthritis was induced by intra-articular injection of monosodium iodoacetate (MIA) into the knee joints of rats. After consumption of PRF001 (10, 50, or 100 mg/kg) for 4 weeks, inflammatory mediators and cytokines in articular cartilage were investigated. In vitro, the levels of inflammatory markers, IL-1ß, TNF-a, COX-2, iNOS, and PGE2, were significantly suppressed by PRF001 treatment. In vivo, the inflammatory mediators and cytokines, IL-1ß, p- Erk1/2, NF-kB, TNF-a, COX-2, and PGE2, as well as MMP3 and MMP7, which have catabolic activity in chondrocytes, were decreased in the MIA-induced osteoarthritic rats following intake of PRF001. Histological analysis revealed that PRF001 had a protective effect on the articular cartilage. Altogether, these results demonstrated that the anti-inflammatory property of PRF001 contributes to its protective effects in osteoarthritis through deregulating IL- 1ß, TNF-a, and subsequent signals, such as p-Erk1/2, NF-κB, COX-2, PGE2, and MMPs.
- Subjects
OSTEOARTHRITIS treatment; RAT diseases; LABORATORY rats; THERAPEUTIC use of cytokines; POLYDEOXYRIBONUCLEOTIDES
- Publication
Korean Journal of Physiology & Pharmacology, 2018, Vol 22, Issue 2, p163
- ISSN
1226-4512
- Publication type
Article
- DOI
10.4196/kjpp.2018.22.2.163