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- Title
Sterile Germination of Oospores of Chara and Seeds of Najas marina.
- Authors
Forsberg, Curt
- Abstract
To obtain sterile sporelings of Chara and seedlings of Najas marina in culture a new method for controlled germination was developed. The special germination requirements of seeds of water plants were satisfied by the use of agar to simulate natural oxygen and redox conditions. Oospores of eight species of Chara and seeds of N. marina were germinated in agar under sterile conditions. Germination of the Chara oospores and Najas seeds did not occur in pure water with redox potentials (E7) between +400 and 500 mV. Addition to the water of reducing agents such as sodium dithionite or thiourea and addition of oxidation-reduction indicator dyes (E7 < +400 mV) promoted the germination of Najas. The oospores of Ch. aculeolata, (with the exception of one collection) Ch. Vulgaris, Ch. hispida, Ch. aspera and Ch. globularis and the fruits of Najas marina were dormant for 1–3 months. The best germination temperature for all of these propagates was 20°–25°C. Light promoted the germination of Chara oospores while the fruits of N. marina germinated better in darkness.
- Subjects
NAJAS marina; AQUATIC plants; GERMINATION; SEED viability; OXIDATION-reduction reaction; EFFECT of temperature on plants; PLANT physiology
- Publication
Physiologia Plantarum, 1965, Vol 18, Issue 1, p128
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1965.tb06875.x