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- Title
Characterization of New Allelic Combinations for High-Oleic Soybeans.
- Authors
Sweeney, Daniel W.; Carrero-Colón, Militza; Hudson, Karen A.
- Abstract
A soybean line with elevated levels of oleic acid was identified in a forward genetic screen. The causative mutation was identified and the line contained a C to T transition mutation causing a novel amino acid substitution in FAD2-1B, a gene that encodes an enzyme critical for soybean fatty acid biosynthesis. Alone, this fad2-1b mutation resulted in a 30% increase in seed oleic acid levels. Double mutant combinations with four previously isolated mutant alleles of the FAD2-1A gene were produced to determine the synergistic effects of mutation in both genes on oleic acid levels in seeds. In all instances, mutant alleles of FAD2-1A and FAD2-1B combined to produce seed containing over 70% oleic acid in the oil fraction. These lines provide a new germplasm resource that could be used to create high oleic soybeans through conventional breeding approaches.
- Subjects
CROP genetics; SOYBEAN; PLANT mutation; OLEIC acid
- Publication
Crop Science, 2017, Vol 57, Issue 2, p611
- ISSN
0011-183X
- Publication type
Article
- DOI
10.2135/cropsci2015.09.0596