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- Title
THE INFLUENCE OF SUBSTRATE AND TEMPERATURE ON LIPASE ACTIVITY IN SOME PLANT SPECIES.
- Authors
AVRAMIUC, MARCEL
- Abstract
The purpose of this work was to study the lipases coming from six plant sources, to see to what extent the substrate (oil) and/or temperature influence the activity of this enzyme, and which of these sources shows a higher activity. The biological materials, used as lipase sources (provided by Suceava Genebank and by Suceava Agricultural Research and Development Station), have been represented by seeds belonging to the following plant species: sunflower (Helianthus annuus L., hd. Rapid), pumpkin (Cucurbita maxima L., local variety), soy (Glycine max L., var. Turda 6114), peanut (Arachis hypogaea L., var. Dabuleni), corn (Zea mays L., hd. F 376), and walnut (Juglans regia L., var. Bratia). As substrates for enzyme activity, were used the following refined oils: sunflower, pumpkin, soy bean, peanut, corn, and walnut, purchased from supermarkets. The research method has consisted in titrating (with a solution of KOH 0.01 N) of fatty acids released from oils by lipase, in a certain time interval. At pH 5.4 and 20°C the lipase from sunflower seeds has registered the highest activity on soybean oil. At the same pH, but at 40°C, the lipase from walnut kernels has registered the highest activity on walnut oil. On its own substrate, the highest activity was registered, at 20°C, by walnut and sunflower lipase, while at 40°C by walnut lipase. It seems that a higher content of linoleic and linolenic acids of some oils (used as substrates), as well as the enzyme chemical composition and spatial configuration caused an increased activity of sunflower and walnut lipase, as compared to the other enzyme sources analyzed.
- Subjects
LIPASES; LINOLENIC acids; PLANT enzyme analysis
- Publication
Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Iasi. Sectiunea II A, Genetica si Biologie Moleculara, 2016, Vol 17, Issue 4, p175
- ISSN
1582-3571
- Publication type
Article