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- Title
The Combination of Berberine, Tocotrienols and Coffee Extracts Improves Metabolic Profile and Liver Steatosis by the Modulation of Gut Microbiota and Hepatic miR-122 and miR-34a Expression in Mice.
- Authors
Cossiga, Valentina; Lembo, Vincenzo; Nigro, Cecilia; Mirra, Paola; Miele, Claudia; D'Argenio, Valeria; Leone, Alessia; Mazzone, Giovanna; Veneruso, Iolanda; Guido, Maria; Beguinot, Francesco; Caporaso, Nicola; Morisco, Filomena; Dallio, Marcello; Ramos-Romero, Sara
- Abstract
Non-alcoholic-fatty liver disease (NAFLD) is spreading worldwide. Specific drugs for NAFLD are not yet available, even if some plant extracts show beneficial properties. We evaluated the effects of a combination, composed by Berberis Aristata, Elaeis Guineensis and Coffea Canephora, on the development of obesity, hepatic steatosis, insulin-resistance and on the modulation of hepatic microRNAs (miRNA) levels and microbiota composition in a mouse model of liver damage. C57BL/6 mice were fed with standard diet (SD, n = 8), high fat diet (HFD, n = 8) or HFD plus plant extracts (HFD+E, n = 8) for 24 weeks. Liver expression of miR-122 and miR-34a was evaluated by quantitativePCR. Microbiome analysis was performed on cecal content by 16S rRNA sequencing. HFD+E-mice showed lower body weight (p < 0.01), amelioration of insulin-sensitivity (p = 0.021), total cholesterol (p = 0.014), low-density-lipoprotein-cholesterol (p < 0.001), alanine-aminotransferase (p = 0.038) and hepatic steatosis compared to HFD-mice. While a decrease of hepatic miR-122 and increase of miR-34a were observed in HFD-mice compared to SD-mice, both these miRNAs had similar levels to SD-mice in HFD+E-mice. Moreover, a different microbial composition was found between SD- and HFD-mice, with a partial rescue of dysbiosis in HFD+E-mice. This combination of plant extracts had a beneficial effect on HFD-induced NAFLD by the modulation of miR-122, miR-34a and gut microbiome.
- Subjects
THERAPEUTIC use of coffee; LIVER disease treatment; THERAPEUTIC use of vitamin E; THERAPEUTIC use of alkaloids; THERAPEUTIC use of plant extracts; BIOLOGICAL models; LIPOPROTEINS; SEQUENCE analysis; BODY weight; FATTY liver; GUT microbiome; ANIMAL experimentation; MICRORNA; RNA; GENE expression; ALANINE; TREATMENT effectiveness; DESCRIPTIVE statistics; COMBINED modality therapy; POLYMERASE chain reaction; CECUM diseases; AMINOTRANSFERASES; MICE; INSULIN resistance; CHOLESTEROL
- Publication
Nutrients, 2021, Vol 13, Issue 4, p1281
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu13041281