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- Title
Shadow imaging for panoptical visualization of brain tissue in vivo.
- Authors
Dembitskaya, Yulia; Boyce, Andrew K. J.; Idziak, Agata; Pourkhalili Langeroudi, Atefeh; Arizono, Misa; Girard, Jordan; Le Bourdellès, Guillaume; Ducros, Mathieu; Sato-Fitoussi, Marie; Ochoa de Amezaga, Amaia; Oizel, Kristell; Bancelin, Stephane; Mercier, Luc; Pfeiffer, Thomas; Thompson, Roger J.; Kim, Sun Kwang; Bikfalvi, Andreas; Nägerl, U. Valentin
- Abstract
Progress in neuroscience research hinges on technical advances in visualizing living brain tissue with high fidelity and facility. Current neuroanatomical imaging approaches either require tissue fixation (electron microscopy), do not have cellular resolution (magnetic resonance imaging) or only give a fragmented view (fluorescence microscopy). Here, we show how regular light microscopy together with fluorescence labeling of the interstitial fluid in the extracellular space provide comprehensive optical access in real-time to the anatomical complexity and dynamics of living brain tissue at submicron scale. Using several common fluorescence microscopy modalities (confocal, light-sheet and 2-photon microscopy) in mouse organotypic and acute brain slices and the intact mouse brain in vivo, we demonstrate the value of this straightforward 'shadow imaging' approach by revealing neurons, microglia, tumor cells and blood capillaries together with their complete anatomical tissue contexts. In addition, we provide quantifications of perivascular spaces and the volume fraction of the extracellular space of brain tissue in vivo. Brain morphology is complex, heterogenous and miniaturized—and notoriously difficult to visualize. Dembitskaya et al. show how fluorescence 'shadow imaging' gives detailed and comprehensive access to the cellular architecture of the mouse brain in vivo.
- Subjects
CAPILLARIES; EXTRACELLULAR space; CELL sheets (Biology); FLUORESCENCE microscopy; MAGNETIC resonance imaging; TISSUE fixation (Histology); MICROSCOPY
- Publication
Nature Communications, 2023, Vol 14, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-023-42055-2