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- Title
Occurrence of ESBL-Producing Enterobacteriaceae in Cubed Beef Samples.
- Authors
ÖNDEŞ, Nilüfer; ÖZPINAR, Haydar
- Abstract
Extended spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae is becoming a worldwide concern for the public health. They can adversely affect the treatment based on modern beta-lactam antibiotics against bacterial infections as well as possibly becoming a source for the spread of ESBL-encoding genes among different and/or the same bacterial species. The objective of this study was to determine the occurrence of ESBL-producing Enterobacteriaceae from a total of 110 cubed beef samples sold in istanbul. The samples were initially homogenized in Enterobacteriaceae broth. Subsequently, the homogenized suspensions were exposed to pre-enrichment at 37°C for 18-24 h. After that, Chromogenic ESBL agar was used for selective enrichment at 37°C for 18-24 h again. The presumptive ESBL-producers were sub-cultured on Trypton Soy agar, followed by an overnight incubation at 37°C. The isolates were identified by Vitek®MS (bioMerieux). Finally, the identified isolates were subjected to agar disc diffusion testing, disc diffusion confirmation testing and MIC determination testing using a combination of cefotaxime (CTX), ceftazidime (CAZ), and cefpodoxime (CPD) discs ± clavulanic acid (CV) according to the guidelines by Clinical and Laboratory Standards Institute. The results revealed that a total of 23 isolates were confirmed to be positive for ESBL-production. Of 23 isolates, the most common species was determined as E. coli (30%), followed by C. brakii (22%), E. cloacae (17%), K. pneumoniae (9%), C. freundli (9%), S. fonticola (4%), K. intermedia (4%), and M. wisconsensis (4%). In conclusion, the results of this study provided that the beef samples harbored ESBL-producing Enterobacteriaceae, and they may, therefore, hold a potential risk for the colonization of the consumers with ESBL-producers.
- Subjects
BETA-lactamase inhibitors; ENZYME inhibitors; ENTEROBACTERIACEAE; MEAT; BEEF; GRAM-negative bacteria
- Publication
Kafkas Universitesi Veteriner Fakultesi Dergisi, 2016, Vol 22, Issue 1, p79
- ISSN
1300-6045
- Publication type
Article
- DOI
10.9775/kvfd.2015.13944