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- Title
Optimization of cold-adapted amylases and protease production by psychrotrophic Streptomyces 4 Alga using response surface methodology.
- Authors
Cotârlet, Mihaela; Bahrim, Gabriela-Elena
- Abstract
Response surface methodology (RSM) employing central composite design (CCD) was used to optimize the fermentation medium constituents for co-production of cold-adapted amylases (α and β amylase) and protease from newly psyhrotrophic Streptomyces 4 Alga under submerged fermentation (SmF). A 24 full factorial central composite design was chosen to explain the combined effects of the four medium constituents; starch and glycerol as carbon sources and sodium caseinate and urea as nitrogen sources. The independent variables were chosen using "one-variable-at-a-time" conventional approach. A linear model, response surface method and the numerical optimization showed that the optimum conditions for cold-adapted α-amylase production (starch 0.75% (w/v), glycerol 0.75% (v/v), sodium caseintate 0.75% (w/v) and urea 0.26% (w/v)) results in 1.71-fold improvement in cold-adapted α-amylase production after 24 h of submerged cultivation, at 7.276xg. For beta-amylase a higher production was not obtained. Instead, in the protease case a higher production was obtained but the concentration was still too small to be industrially useful. The studied enzyme may have tremendous applications in food industry, detergents in processes that are running at lower temperature.
- Subjects
AMYLASES; PROTEOLYTIC enzymes; FERMENTATION; STREPTOMYCES; GLYCERIN; FOOD industry
- Publication
Turkish Journal of Biochemistry / Turk Biyokimya Dergisi, 2011, Vol 36, Issue 2, p83
- ISSN
0250-4685
- Publication type
Article