We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Mitochondrial DNA copy number and hnRNP A2/B1 protein: Biomarkers for direct exposure of benzene.
- Authors
Eom, Ha-Young; Kim, Hye-Ran; Kim, Hwan-Young; Han, Dong-Kyun; Baek, Hee-Jo; Lee, Jae-Hyuk; Moon, Jai Dong; Shin, Jong-Hee; Suh, Soon-Pal; Ryang, Dong-Wook; Kook, Hoon; Shin, Myung-Geun
- Abstract
The present study was performed to identify biomarkers for exposure of benzene in blood cells and hematopoietic tissues. Peripheral mononuclear cells, hematopoietic stem cells, and leukemia cell lines were cultured in RPMI 1640 media with the addition of 0, 1, and 10 mM of benzene. Hydrogen peroxide was measured using an enzyme immunoassay. Mitochondrial mass, membrane potential, and mitochondrial DNA (mtDNA) copy number were measured using MitoTracker Green/Red probes, and real-time polymerase chain reaction. In addition, two-dimensional gel electrophoresis and mass spectrometry matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) technology were performed to identify protein markers. The mitochondrial contents and membrane potentials were dramatically increased after three weeks of direct benzene exposure. The hydrogen peroxide level increased significantly after two weeks of treatment with benzene (4.4 ± 1.9 µM/mg protein) compared to the non-benzene treatment group (1.2 ± 1.0; p = 0.001). The mtDNA copy number gradually increased after exposure to benzene. Numerous protein markers showed significant aberrant expression after exposure to benzene. Among them, the heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 was markedly decreased after exposure to benzene. Thus, increased mitochondrial mass, mtDNA copy number, and the hnRNP A2/B1 protein were biomarkers for benzene-related toxicity and hematotoxicity. Environ. Toxicol. Chem. 2011;30:2762-2770. © 2011 SETAC
- Subjects
MITOCHONDRIAL DNA; BIOMARKERS; CELL lines; STEM cells; HYDROGEN peroxide
- Publication
Environmental Toxicology & Chemistry, 2011, Vol 30, Issue 12, p2762
- ISSN
0730-7268
- Publication type
Article
- DOI
10.1002/etc.675