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- Title
Serum alpha<sub>1</sub>-proteinase inhibitor concentrations in healthy dogs - method validation and determination of reference interval and intra-individual variation.
- Authors
Heilmann, Romy M.; Ruaux, Craig G.; Burgener, Iwan A.; Hern, Jennifer D.; Suchodolski, Jan S.; Steiner, Jörg M.
- Abstract
Background A chronic loss of canine α1-proteinase inhibitor (cα1- PI) into the gastrointestinal ( GI) tract could change the systemic proteinase-proteinase inhibitor balance. Serum cα1- PI concentrations have not been studied in dogs with well-defined GI diseases. Objectives To further evaluate serum cα1- PI concentrations in dogs with GI diseases, the objectives of this study were to (1) analytically validate a previously developed fecal cα1- PI immunoassay to determine serum concentrations, (2) determine a population-based reference interval ( RI) and assess the clinical utility, (3) determine stability of serum cα1- PI, (4) determine the intra-individual variation in healthy dogs, and (5) determine the clinically relevant magnitude of change of serum cα1- PI. Methods Prestudy validation of the 125I-cα1- PI immunoassay included linearity, spiking recovery, and intra- and inter-assay precision. A RI was calculated with samples of healthy dogs. Stability at −20°C was tested on 36 samples. Intra-individual variation was assessed using samples collected from 11 healthy dogs over a 12-week period. Results The cα1- PI radioimmunoassay (RIA) was linear, accurate, precise, and reproducible. Serum cα1- PI decreased by 11% after one year at −20°C. Analytical, intra-individual, inter-individual, and total variation were 6.4, 9.9, 9.0, and 25.3%, respectively. The RI for serum cα1- PI was 732-1802 mg/L ( n = 87); there were no differences between sex and age groups. The index of individuality was 1.31. As analytical variation was > ½ inter-individual variation, the minimum critical difference was not determined. Conclusions The results of this study provide the basis for further evaluating serum cα1- PI in dogs with GI disease. Using a population-based RI for serum cα1- PI appears to be appropriate.
- Subjects
PROTEINASES; DOG diseases; GASTROINTESTINAL diseases; PROTEOLYTIC enzymes; RADIOIMMUNOASSAY
- Publication
Veterinary Clinical Pathology, 2013, Vol 42, Issue 2, p190
- ISSN
0275-6382
- Publication type
Article
- DOI
10.1111/vcp.12039