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- Title
Virulence traits in species isolated from different sources.
- Authors
Cruz, Ariadnna; Xicohtencatl-Cortes, Juan; González-Pedrajo, Bertha; Bobadilla, Miriam; Eslava, Carlos; Rosas, Irma
- Abstract
spp. () includes gram-negative opportunistic foodborne pathogens known as rare but important causes of life-threatening neonatal infections. However, the pathogenic mechanism is not yet clear. In this study, 43 isolates of Cronobacter, from human and nonhuman sources, were analyzed. A total of four clusters were identified and 32 DNA pulsotypes were observed by pulsed-field gel electrophoresis. In addition, 86% of the Cronobacter isolates were able to adhere to HEp-2 cells and 35% were invasive, isolates being the most efficient. Twenty-six percent of Cronobacter isolates were able to form biofilms, mainly those from nonhuman sources, such as and . Three putative virulence genes (siderophore-interacting protein ( sip), type III hemolysin ( hly), and plasminogen activator ( cpa)) were identified by bioinformatic analysis and then detected by PCR. The sip gene was the most frequently detected (60%; 26/43), followed by the hly gene (37%; 16/43) and the cpa gene (28%; 12/43). The three genes were identified primarily in C. sakazakii. Our data show that Cronobacter species harbor different virulence traits.
- Subjects
GRAM-negative bacteria; MICROBIAL virulence; FOOD pathogens; PULSED-field gel electrophoresis; DNA; BIOFILMS; BIOINFORMATICS; GENETICS; BACTERIAL typing
- Publication
Canadian Journal of Microbiology, 2011, Vol 57, Issue 9, p735
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/w11-063