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- Title
Prospective study applying spoligotyping directly to DNA from sputum samples of patients suspected of having tuberculosis.
- Authors
Cafrune, Patricia Izquierdo; Possuelo, Lia Gonçalves; Ribeiro, Andrezza Wolowski; Ribeiro, Marta Osório; Unis, Gisela; Jarczewski, Carla Adriane; Rossetti, Maria Lúcia Rosa; Zaha, Arnaldo
- Abstract
A prospective study was designed to evaluate the clinical usefulness of spoligotyping applied directly to sputum samples. Patients suspected of having tuberculosis were recruited at the Hospital Sanatorio Partenon in Porto Alegre, Brazil. Of the 197 samples included in the analysis, 175 (88.8%) yielded a spoligotyping result that fully matched that obtained from culture. Low bacillary samples presented lower accuracy (50%). From 135 Mycobacterium tuberculosis spoligopatterns, we identified 44 different spoligotypes, of which 21 were shared patterns and 23 were unique. T1 was the most frequent subfamily. The genotyping strategy proposed here presents a short turnaround time and could be helpful in providing rapid information on strain identities in a clinical setting.
- Subjects
MYCOBACTERIUM tuberculosis; MYCOBACTERIAL diseases; TUBERCULOSIS; SPUTUM; DNA
- Publication
Canadian Journal of Microbiology, 2009, Vol 55, Issue 7, p895
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W09-033