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- Title
Rapid discrimination among individual DNA hairpin molecules at single-nucleotide resolution using an ion channel.
- Authors
Vercoutere, Wenonah; Winters-Hilt, Stephen; Olsen, Hugh; Deamer, David; Haussler, David; Akeson, Mark
- Abstract
RNA and DNA strands produce ionic current signatures when driven through an a-hemolysin channel by an applied voltage. Here we combine this nanopore detector with a support vector machine (SVM) to analyze DNA hairpin molecules on the millisecond time scale. Measurable properties include duplex stem length, base pair mismatches, and loop length. This nanopore instrument can discriminate between individual DNA hairpins that differ by one base pair or by one nucleotide.
- Subjects
ION channels; DNA
- Publication
Nature Biotechnology, 2001, Vol 19, Issue 3, p248
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/85696