We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Myogenic expression of an injectable protease-resistant growth hormone?releasing hormone augments long-term growth in pigs.
- Authors
Draghia-Akli, Ruxandra; Fiorotto, Marta L.; Hill, Leigh Anne; Malone, P. Brandon; Deaver, Daniel R.; Schwartz, Robert J.
- Abstract
Ectopic expression of a new serum protease-resistant porcine growth hormone-releasing hormone, directed by an injectable muscle-specific synthetic promoter plasmid vector (pSP-HV-GHRH), elicits growth in pigs. A single 10 mg intramuscular injection of pSP-HV-GHRH DNA followed by electroporation in three-week-old piglets elevated serum GHRH levels by twofold to fourfold, enhanced growth hormone secretion, and increased serum insulin-like growth factor-I by threefold to sixfold over control pigs. After 65 days the average body weight of the pigs injected with pSP-HV-GHRH was ∼37% greater than the placebo-injected controls and resulted in a significant reduction in serum urea concentration, indicating a decrease in amino acid catabolism. Evaluation of body composition indicated a uniform increase in mass, with no organomegaly or associated pathology.
- Subjects
GROWTH hormone releasing factor; GENE therapy; ELECTROPORATION
- Publication
Nature Biotechnology, 1999, Vol 17, Issue 12, p1179
- ISSN
1087-0156
- Publication type
Article
- DOI
10.1038/70718