We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Overexpression of SUMO perturbs the growth and development of Caenorhabditis elegans.
- Authors
Rytinki, Miia; Lakso, Merja; Pehkonen, Petri; Aarnio, Vuokko; Reisner, Kaja; Peräkylä, Mikael; Wong, Garry; Palvimo, Jorma
- Abstract
Small ubiquitin-related modifiers (SUMOs) are important regulator proteins. Caenorhabditis elegans contains a single SUMO ortholog, SMO-1, necessary for the reproduction of C. elegans. In this study, we constructed transgenic C. elegans strains expressing human SUMO-1 under the control of pan-neuronal ( aex- 3) or pan-muscular ( myo- 4) promoter and SUMO-2 under the control of myo- 4 promoter. Interestingly, muscular overexpression of SUMO-1 or -2 resulted in morphological changes of the posterior part of the nematode. Movement, reproduction and aging of C. elegans were perturbed by the overexpression of SUMO-1 or -2. Genome-wide expression analyses revealed that several genes encoding components of SUMOylation pathway and ubiquitin-proteasome system were upregulated in SUMO-overexpressing nematodes. Since muscular overexpression of SMO-1 also brought up reproductive and mobility perturbations, our results imply that the phenotypes were largely due to an excess of SUMO, suggesting that a tight control of SUMO levels is important for the normal development of multicellular organisms.
- Subjects
CAENORHABDITIS elegans; GENE expression; DEVELOPMENTAL biology; IMMUNOMODULATORS; UBIQUITIN; GENETIC regulation; REPRODUCTION; AGING
- Publication
Cellular & Molecular Life Sciences, 2011, Vol 68, Issue 19, p3219
- ISSN
1420-682X
- Publication type
Article
- DOI
10.1007/s00018-011-0627-4