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- Title
Staining tissue with methacrylate casts for light microscopy.
- Authors
Sekosan, Marin; Schraufnagel, Dean E.
- Abstract
Scanning electron microscopy (SEM) of methyl methacrylate casts and light microscopy (LM) of tissue are well-established methods for studying the microcirculation. The two are complimentary, but methacrylate is transparent and thus its presence is often not appreciated by LM. Histologic stains applied to methyl methacrylate in tissue sections would better identify by LM and allow the relationships with the SEM view of cast vasculature. We sought to test different stains on cast tissue to find one that would accent the cast. Surgically removed and autopsied human lungs were cast with methacry late and processed by routine light microscopic methods. They were stained with the hematoxylin and eosin, Masson trichome, elastic-van Gieson, Grocottmethenamine silver, Brown-Brennan, and Ziehl-Neelsen methods. The Ziehl-Neelsen procedure stained the methacry late best, giving it a red color. This procedure also worked well without heating. We conclude that (1) cast methacry late lung can be processed for routine LM with excellent results; (2) methacry late stains well with the Ziehl-Neelsen technique; (3) the acid-fast stained cast lung shows capillaries and cells in both normal and diseased lung better than the routine hematoxylin and eosin stain; (4) this technique can be used to assess filling and correlate findings on the same tissue with the two different microscopic methods.
- Publication
Scanning, 1998, Vol 20, Issue 7, p508
- ISSN
0161-0457
- Publication type
Article
- DOI
10.1002/sca.1998.4950200704