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- Title
Caffeic acid‐grafted chitooligosaccharides downregulate MAPK and NF‐kB in RAW264.7 cells.
- Authors
Bui, Van‐Hoai; Vo, Hong‐Tham N.; Kim, Se‐Kwon; Ngo, Dai‐Nghiep
- Abstract
Chitooligosaccharide (COS) is a derivative of chitosan, which is a natural macromolecular compound. COS has been shown effects in an inflammatory response. Recent reports show that COS derivatives have enhanced anti‐inflammatory activity by inhibiting intracellular signals. Evaluation of the anti‐inflammatory effect of caffeic acid conjugated COS chain (CA‐COS) was performed in this study. The effects of CA‐COS on the inflammatory response were demonstrated in lipopolysaccharide‐stimulated RAW264.7 macrophages. The results showed that CA‐COS inhibited nitric oxide (NO) production and downregulated the gene expression of nitric oxide synthase (iNOS), and cytokines such as tumor necrosis factor‐alpha (TNF‐α), IL‐1β, and IL‐6 without cytotoxic effect. In addition, western blot analysis showed that CA‐COS inhibits the protein expression of iNOS and nuclear factor kappa B (NF‐kB), including p50 and p65, and mitogen‐activated protein kinase (MAPK) signaling pathways. Collectively, these results provide clear evidence for the anti‐inflammatory mechanism of CA‐COS that show great potential as a novel agent for the prevention and therapy of inflammatory diseases.
- Subjects
CAFFEIC acid; TUMOR necrosis factors; NF-kappa B; MITOGEN-activated protein kinases; NITRIC-oxide synthases; WESTERN immunoblotting
- Publication
Chemical Biology & Drug Design, 2024, Vol 103, Issue 3, p1
- ISSN
1747-0277
- Publication type
Article
- DOI
10.1111/cbdd.14496