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- Title
Detection and cloning of expressed sequences linked to a target gene.
- Authors
Vallejos, C. E.; Malandro, J. J.; Sheehy, K.; Zimmermann, M. J.
- Abstract
DNA markers tightly linked to a target gene are essential starting points for positional cloning. We combined ”differential display of mRNA” and ”bulked segregant analysis” in order to detect and clone ten expressed sequences as markers linked to a virus resistance gene in Phaseolus vulgaris. The combination of these two procedures could be used in lieu of positional cloning, provided polymorphisms detectable by differential display exist in the target gene. Isolation of expressed sequences from specific chromosome regions can also be accomplished by combining these procedures.
- Subjects
COMMON bean; BEAN common mosaic virus; PLANT viruses; BEAN diseases &; pests
- Publication
Theoretical & Applied Genetics, 2000, Vol 101, Issue 7, p1109
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s001220051586