We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Modification of surfactant protein D by reactive oxygen-nitrogen intermediates is accompanied by loss of aggregating activity, in vitro and in vivo.
- Authors
Matalon, Sadis; Shrestha, Kedar; Kirk, Marion; Waldheuser, Stephanie; McDonald, Barbara; Smith, Kelly; Zhiqian Gao; Belaaouaj, Abderrazzak; Crouch, Erika C.
- Abstract
Surfactant protein D (SP-D) is an important effector of innate immunity. We have previously shown that SP-D accumulates at sites of acute bacterial infection and neutrophil infiltration, a setting associated with the release of reactive species such as peroxynitrite. Incubation of native SP-D or trimeric SP-D lectin domains (NCRDs) with peroxynitrite resulted in nitration and nondisulfide cross-linking. Modifications were blocked by peroxynitrite scavengers or pH inactivation of peroxynitrite, and mass spectroscopy confirmed nitration, of conserved tyrosine residues within the C-terminal neck and lectin domains. Mutant NCRDs lacking one or more of the tyrosines allowed us to demonstrate preferential nitration of Tyr314 and the formation of Tyr228-dependent cross-links. Although there was no effect of peroxynitrite or tyrosine mutations on lectin activity, incubation of SP-D dodecamers or murine lavage with peroxynitrite decreased the SP-D-dependent aggregation of lipopolysaccharide-coated beads, supporting our hypothesis that defective aggregation results from abnormal cross-linking. We also observed nitration, cross-linking of SP-D, and a significant decrease in SP-D-dependent aggregating activity in the lavage of mice acutely exposed to nitrogen dioxide. Thus, modification of SP-D by reactive oxygen-nitrogen species could contribute to alterations in the structure and function of SP-D at sites of inflammation in vivo.
- Subjects
PROTEINS; REACTIVE oxygen species; NITROGEN; NATURAL immunity; NEUTROPHILS; LECTINS
- Publication
FASEB Journal, 2009, Vol 23, Issue 5, p1415
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.08-120568