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- Title
Glycyrrhizinate attenuates exosome-induced endothelial cell proliferation and permeability through p38 pathway.
- Authors
Shun Xu; Aili Wang; Zaoli Shen; Yu Chen; Qing Jia
- Abstract
Purpose: To investigate the mechanism of action involved in the treatment of severe burn injury with glycyrrhizinate (DG). Methods: Exosomes were purified from sera of patients with burn injury using an ultra-high-speed centrifuge, and verified by western blot. Cell proliferation and permeability were assessed using cell counting kit (CCK)-8, transepithelial-transendothelial electrical resistance (TEER), and Fluorescein Isothiocyanate (FITC) dextran assays, while immunoblotting was used for assay of the levels of p38, occluding, and zonula occludens 1 (ZO-1). Results: Serum-derived exosomes (serum-exo) significantly suppressed cell proliferation while causing hyperpermeability in HUVECs (p < 0.001). Furthermore, DG alleviated the hyperpermeability and inhibition of cell proliferation caused by serum-exo (p < 0.001). In addition, the upregulation of p-P38 induced by serum-exo decreased upon DG treatment. Interestingly, the effect of DG was blocked by anisomycin, a specific p38 activator, indicating that p38 signaling pathway may contribute to the function of DG. Conclusion: Glycyrrhizinate attenuates serum-exo-induced cell proliferation and permeability alteration via p38 signaling pathway, thereby making it a potential agent for the management of severe burn injury.
- Subjects
CELL permeability; CELL proliferation; TIGHT junctions; ENDOTHELIAL cells; FLUORESCEIN isothiocyanate; NEOVASCULARIZATION
- Publication
Tropical Journal of Pharmaceutical Research, 2023, Vol 22, Issue 3, p517
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v22i3.8