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- Title
Bioprospecting for culturable actinobacteria with antimicrobial properties isolated from rivers in Colombian Orinoquia.
- Authors
Pastrana-Camacho, Natalia; Suárez, Zulma; Acosta-González, Alejandro; Arango, Carolina; Haltli, Brad; Correa, Hebelin; Kerr, Russel; Duque, Carmenza; Díaz, Luis Eduardo
- Abstract
Purpose: To Isolate and characterize Actinobacteria with antimicrobial activity from Guaviare River (Colombia). Methods: Water and sediment samples were collected from Guaviare River. Direct plating, heat and CaCO3 methods were used to isolate Actinobacteria. Six bacterial strains were tested using T-Streak method: Escherichia coli ATCC 23724, Staphylococus aureus ATCC 25923, Acinetobacter baumannii ATCC 19606, Bacillus subtilis ATCC 21556, Klebsiella pneumoniae ATCC 700603, Chromobacterium violaceum ATCC 31532. Strains of Fusarium sp. H24, Trichoderma harzianum H5 and Colletotrichum gloeosporioides were tested using Kirby-Bauer method. Isolates with high antimicrobial activity were selected for further taxonomic identification. Results: A total of 374 actinobacteria isolates were obtained. Seven isolates exhibited high antimicrobial activity (p < 0.05) and were confirmed as members of Streptomycetaceae family. Of these, three isolates showed differential phenotypic and genotypic profiles, indicating that they may represent new species. Conclusions: To date, this is the first study of this type in Colombian Orinoquia and indicates that this promising source of Actinobacteria from aquatic sediments with the ability to produce antimicrobial secondary metabolites.
- Subjects
LLANOS (Colombia &; Venezuela); BIOPROSPECTING; BIODIVERSITY research; BODIES of water; BIOPIRACY
- Publication
Tropical Journal of Pharmaceutical Research, 2016, Vol 15, Issue 6, p1259
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v15i6.19