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- Title
Concordancia de dos pruebas serológicas para el diagnóstico de la enfermedad de Chagas.
- Authors
Farfán-García, Ana E.; Castellanos-Domínguez, Yeny Z.; Luna-Marín, Katherine P.; Angulo-Silva, Víctor M.
- Abstract
Objective Establishing agreement between an in-house ELISA and an indirect immunofluorescence (IIF) assay for diagnosing infection caused byT. cruzi using blood eluates. Methodology The study involved an evaluation of diagnostic technology and cross-sectional sampling of 650 people residing in an endemic area of Colombia. The area below the receiver operating characteristic (ROC) curve was determined and IIF was used as gold standard. The cut-off for the ELISA was established, as well as agreement between readings. Results The in-house ELISA had 0.99 agreement (0.989 to 0.992 95%CI) between the two readings taken and ROC curve area was 0.9795. The ELISA test cut-off point was set at 0.5 absorbance; 16.6 % were positive by ELISA and 10.9 % by IIF. Conclusions The in-house ELISA assay had good agreement compared to IIF; it would thus seem a good diagnostic choice for testing a target population living in remote areas.
- Subjects
DIAGNOSIS of Chagas' disease; SEROLOGY; ENZYME-linked immunosorbent assay; IMMUNOFLUORESCENCE; TRYPANOSOMA cruzi; RECEIVER operating characteristic curves; GOLD standard
- Publication
Revista de Salud Pública, 2013, Vol 15, Issue 2, p192
- ISSN
0124-0064
- Publication type
Article