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- Title
Heterologous expression of Aspergillus aculeatus endo-polygalacturonase in Pichia pastoris by high cell density fermentation and its application in textile scouring.
- Authors
Abdulrachman, Dede; Thongkred, Paweena; Kocharin, Kanokarn; Nakpathom, Monthon; Somboon, Buppha; Narumol, Nootsara; Champreda, Verawat; Eurwilaichitr, Lily; Suwanto, Antonius; Nimchua, Thidarat; Chantasingh, Duriya
- Abstract
Background: Removal of non-cellulosic impurities from cotton fabric, known as scouring, by conventional alkaline treatment causes environmental problems and reduces physical strength of fabrics. In this study, an endo-polygalacturonase (EndoPG) from Aspergillus aculeatus produced in Pichia pastoris was evaluated for its efficiency as a bioscouring agent while most current bioscouring process has been performed using crude pectinase preparation. Results: The recombinant EndoPG exhibited a specific activity of 1892.08 U/mg on citrus pectin under the optimal condition at 50 °C, pH 5.0 with a Vmax and Km of 65,451.35 μmol/min/mL and 15.14 mg/mL, respectively. A maximal activity of 2408.70 ± 26.50 U/mL in the culture supernatant was obtained by high cell density batch fermentation, equivalent to a 4.8 times greater yield than that from shake-flask culture. The recombinant enzyme was shown to be suitable for application as a bioscouring agent, in which the wettability of cotton fabric was increased by treatment with enzyme at 300 U/mL scouring solution at 40 °C, pH 5.0 for 1 h. The bio-scoured fabric has comparable wettability to that obtained by conventional chemical scouring, but has higher tensile strength. Conclusion: The work has demonstrated for the first time functions of A. aculeatus EndoPG on bioscouring in eco-textile processing. EndoPG alone was shown to possess effective scouring activity. High expression level and homogeneity could be achieved in bench-scale bioreactor.
- Subjects
COTTON textiles; PICHIA pastoris; WOOL scouring; SCOURING of textiles; NONCELLULOSE organic fiber
- Publication
BMC Biotechnology, 2017, Vol 17, p1
- ISSN
1472-6750
- Publication type
Article
- DOI
10.1186/s12896-017-0334-9