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- Title
Pressure-clamped single-fiber recording technique: A new recording method for studying sensory receptors.
- Authors
Sonekatsu, Mayumi; Yamada, Hiroshi; Gu, Jianguo G
- Abstract
An electrophysiological technique that can record nerve impulses from a single nerve fiber is indispensable for studying modality-specific sensory receptors such as low threshold mechanoreceptors, thermal receptors, and nociceptors. The teased-fiber single-unit recording technique has long been used to resolve impulses that are likely to be from a single nerve fiber. The teased-fiber single-unit recording technique involves tedious nerve separation procedures, causes nerve fiber impairment, and is not a true single-fiber recording method. In the present study, we describe a new and true single-fiber recording technique, the pressure-clamped single-fiber recording method. We have applied this recording technique to mouse whisker hair follicle preparations with attached whisker afferents as well as to skin-nerve preparations made from mouse hindpaw skin and saphenous nerves. This new approach can record impulses from rapidly adapting mechanoreceptors (RA), slowly adapting type 1 mechanoreceptors (SA1), and slowly adapting type 2 mechanoreceptors (SA2) in these tissue preparations. We have also applied the pressure-clamped single-fiber recordings to record impulses on Aβ-fibers, Aδ-fibers, and C-fibers. The pressure-clamped single-fiber recording technique provides a new tool for sensory physiology and pain research.
- Subjects
SENSORY receptors; HAIR care products; ACTION potentials; MECHANORECEPTORS; HAIR follicles
- Publication
Molecular Pain, 2020, Vol 16, p1
- ISSN
1744-8069
- Publication type
Article
- DOI
10.1177/1744806920927852