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- Title
The G-Protein-Coupled Neuropeptide Y Receptor Type 2 is Highly Dynamic in Lipid Membranes as Revealed by Solid-State NMR Spectroscopy.
- Authors
Schmidt, Peter; Thomas, Lars; Müller, Paul; Scheidt, Holger A.; Huster, Daniel
- Abstract
In spite of the recent success in crystallizing several G-protein-coupled receptors (GPCRs), a comprehensive biophysical characterization of these molecules under physiological conditions also requires the study of the molecular dynamics of these proteins. The molecular mobility of the human neuropeptide Y receptor type 2 reconstituted into dimyristoylphosphatidylcholine (DMPC) membranes was investigated by means of solid-state NMR spectroscopy. Static 15N NMR spectra show that the receptor performs axially symmetric motions in the membrane, and several residues undergo large amplitude fluctuations. This was confirmed by quantitative measurements of the motional 1H,13C order parameter of the CH, CH2, and CH3 groups. In directly polarized 13C NMR experiments, these order parameters showed astonishingly low values of SCH=0.55, S
- Subjects
PROTEIN structure; NEUROPEPTIDE Y receptors; BILAYER lipid membranes; G protein coupled receptors; NUCLEAR magnetic resonance spectroscopy
- Publication
Chemistry - A European Journal, 2014, Vol 20, Issue 17, p4986
- ISSN
0947-6539
- Publication type
Article
- DOI
10.1002/chem.201304928