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- Title
Application of nested PCR for diagnosis of histoplasmosis.
- Authors
Ohno, Hideaki; Tanabe, Koichi; Umeyama, Takashi; Kaneko, Yukihiro; Yamagoe, Satoshi; Miyazaki, Yoshitsugu
- Abstract
Histoplasmosis is a fungal infection that, although not endemic in Japan, has seen a rise in the number of Japanese cases since the mid-1980s. Diagnosis of the disease is not straightforward, and the main method of detection, fungal culture (which has biosafety-related issues), is of low sensitivity in general. Alternative methods that depend on antibody or antigen detection have had limited use. We have developed a histoplasmosis detection method based on PCR amplification of the Histoplasma capsulatum M antigen gene. We compared this method with fungal culture and serological diagnostic techniques. Among five cases that were finally diagnosed as histoplasmosis, the fungal culture method was only successful in identifying one such case. Although the presence of anti- H. capsulatum antibodies was confirmed in three cases, our PCR method identified four of five cases of histoplasmosis. The performance of our PCR method could not be compared with the antigen detection method, which is used in the United States but is not routinely used in Japan. However, the PCR method was shown to have high sensitivity and specificity for H. capsulatum. Although the number of histoplasmosis cases examined in this study was small, our data suggest that the molecular diagnosis technique has potential for increasing the reliability of histoplasmosis diagnosis when used in combination with established methods.
- Subjects
POLYMERASE chain reaction; HISTOPLASMOSIS diagnosis; MYCOSES; JAPANESE people; HISTOPLASMOSIS; FUNGAL cultures; BIOSAFETY; DISEASES
- Publication
Journal of Infection & Chemotherapy (Springer Science & Business Media B.V.), 2013, Vol 19, Issue 5, p999
- ISSN
1341-321X
- Publication type
Article
- DOI
10.1007/s10156-013-0548-2