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- Title
Exploring the mechanism and efficient use of a durable gene-mediated resistance to bacterial blight disease in rice.
- Authors
Deng, Yong; Liu, Hongbo; Zhou, Yan; Zhang, Qinglu; Li, Xianghua; Wang, Shiping
- Abstract
<italic>Xanthomonas oryzae</italic> pv. <italic>oryzae</italic> (<italic>Xoo</italic>) causes bacterial blight, the most devastating bacterial disease of rice worldwide. The major disease resistance gene <italic>Xa3/Xa26</italic> confers a durable resistance to <italic>Xoo</italic> with a dosage effect. However, the mechanism of <italic>Xa3/Xa26</italic>-mediated resistance remains to be elucidated. We created near-isogenic lines carrying <italic>Xa3/Xa26</italic> with a background of <italic>indica</italic> and <italic>japonica</italic>, the two major subspecies of Asian cultivated rice. Analyzing these rice lines showed that the <italic>japonica</italic> background facilitated resistance to <italic>Xoo</italic>, which was associated with increased <italic>Xa3/Xa26</italic> expression, compared with rice lines with an <italic>indica</italic> background. This characteristic of <italic>Xa3/Xa26</italic> was related to the <italic>WRKY45</italic> locus, which had higher expression with the <italic>japonica</italic> background than with the <italic>indica</italic> background. However, the two alleles of the <italic>WRKY45</italic> locus had different expression levels, with the <italic>WRKY45-1</italic> expression level being higher than that of <italic>WRKY45-2</italic> for both <italic>japonica</italic> and <italic>indica</italic> backgrounds<italic>.</italic> In addition, the resistance level conferred by <italic>Xa3/Xa26</italic> was higher in the presence of <italic>WRKY45-1</italic> than in the presence of <italic>WRKY45-2</italic> for both <italic>japonica</italic> and <italic>indica</italic> backgrounds. <italic>Xa3/Xa26</italic>-mediated resistance was associated with increased accumulation of jasmonic acid (JA), JA-isoleucine, and terpenoid and flavonoid phytoalexins. Exogenous JA application enhanced <italic>Xa3/Xa26</italic>-mediated resistance. These results not only provide more knowledge toward understanding the mechanism of <italic>Xa3/Xa26</italic>-mediated resistance but also offer the best choice for using <italic>Xa3/Xa26</italic> for rice resistance improvement, specifically, a <italic>japonica</italic> background with the <italic>WRKY45-1</italic> allele.
- Subjects
DISEASE resistance of plants; RICE disease &; pest resistance; RICE genetics; XANTHOMONAS oryzae; GENE expression in plants
- Publication
Molecular Breeding, 2018, Vol 38, Issue 2, p0
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-018-0778-1