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- Title
Cholesterol Alters the Phase Separation in Model Membranes Containing hBest1.
- Authors
Videv, Pavel; Mladenova, Kirilka; Andreeva, Tonya D.; Park, Jong Hun; Moskova-Doumanova, Veselina; Petrova, Svetla D.; Doumanov, Jordan A.
- Abstract
Human retinal pigment epithelial (RPE) cells express the transmembrane Ca2+-dependent Cl− channel bestrophin-1 (hBest1) of the plasma membrane. Mutations in the hBest1 protein are associated with the development of distinct pathological conditions known as bestrophinopathies. The interactions between hBest1 and plasma membrane lipids (cholesterol (Chol), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) and sphingomyelin (SM)) determine its lateral organization and surface dynamics, i.e., their miscibility or phase separation. Using the surface pressure/mean molecular area (π/A) isotherms, hysteresis and compressibility moduli (Cs−1) of hBest1/POPC/Chol and hBest1/SM/Chol composite Langmuir monolayers, we established that the films are in an LE (liquid-expanded) or LE-LC (liquid-condensed) state, the components are well-mixed and the Ca2+ ions have a condensing effect on the surface molecular organization. Cholesterol causes a decrease in the elasticity of both films and a decrease in the ΔGmixπ values (reduction of phase separation) of hBest1/POPC/Chol films. For the hBest1/SM/Chol monolayers, the negative values of ΔGmixπ are retained and equalized with the values of ΔGmixπ in the hBest1/POPC/Chol films. Shifts in phase separation/miscibility by cholesterol can lead to changes in the structure and localization of hBest1 in the lipid rafts and its channel functions.
- Subjects
MEMBRANE separation; PHASE separation; MEMBRANE lipids; CHOLESTEROL; BLOOD lipids; LIPID rafts; CHLORIDE channels; CALCIUM channels
- Publication
Molecules, 2022, Vol 27, Issue 13, p4267
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27134267