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- Title
Fluorescent Immunoassay with a Copper Polymer as the Signal Label for Catalytic Oxidation of O -Phenylenediamine.
- Authors
Feng, Yunxiao; Liu, Gang; Zhang, Chunhuan; Li, Jinrui; Li, Yuanyuan; Liu, Lin
- Abstract
This work suggested that Cu2+ ion coordinated by the peptide with a histidine (His or H) residue in the first position from the free N-terminal reveals oxidase-mimicking activity. A biotinylated polymer was prepared by modifying His residues on the side chain amino groups of lysine residues (denoted as KH) to chelate multiple Cu2+ ions. The resulting biotin-poly-(KH-Cu)20 polymer with multiple catalytic sites was employed as the signal label for immunoassay. Prostate specific antigen (PSA) was determined as the model target. The captured biotin-poly-(KH-Cu)20 polymer could catalyze the oxidation of o-phenylenediamine (OPD) to produce fluorescent 2,3-diaminophenazine (OPDox). The signal was proportional to PSA concentration from 0.01 to 2 ng/mL, and the detection limit was found to be eight pg/mL. The high sensitivity of the method enabled the assays of PSA in real serum samples. The work should be valuable for the design of novel biosensors for clinical diagnosis.
- Subjects
CATALYTIC oxidation; PROSTATE-specific antigen; IMMUNOASSAY; AMINO group; COPPER; HISTIDINE; POLYMERS
- Publication
Molecules, 2022, Vol 27, Issue 12, p3675
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules27123675