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- Title
Study of Osteocyte Behavior by High-Resolution Intravital Imaging Following Photo-Induced Ischemia.
- Authors
Yuan, Hengfeng; Jiang, Wen; Chen, Yuanxin; Kim, Betty Y. S.; Teng, Lesheng; Yang, Zhaogang; Xie, Jing
- Abstract
Ischemic injuries and local hypoxia can result in osteocytes dysfunction and play a key role in the pathogenesis of avascular osteonecrosis. Conventional imaging techniques including magnetic resonance imaging (MRI) and computed tomography (CT) can reveal structural and functional changes within bony anatomy; however, characterization of osteocyte behavioral dynamics in the setting of osteonecrosis at the single cell resolution is limited. Here, we demonstrate an optical approach to study real-time osteocyte functions in vivo. Using nicotinamide adenine dinucleotide (NADH) as a biomarker for metabolic dynamics in osteocytes, we showed that NADH level within osteocytes transiently increase significantly after local ischemia through non-invasive photo-induced thrombosis of afferent arterioles followed by a steady decline. Our study presents a non-invasive optical approach to study osteocyte behavior through the modulation of local environmental conditions. Thus it provides a powerful toolkit to study cellular processes involved in bone pathologies in vivo.
- Subjects
OSTEOCYTES; HYPOXEMIA; MAGNETIC resonance imaging; ISCHEMIA; MOLECULAR dynamics; BIOACTIVE compounds
- Publication
Molecules, 2018, Vol 23, Issue 11, p2874
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules23112874