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- Title
Anti-Inflammatory Activity of a Polymeric Proanthocyanidin from Serjania schiedeana.
- Authors
Salinas-Sánchez, David Osvaldo; Jiménez-Ferrer, Enrique; Sánchez-Sánchez, Veronica; Zamilpa, Alejandro; González-Cortazar, Manasés; Tortoriello, Jaime; Herrera-Ruiz, Maribel
- Abstract
The ethyl acetate extract (SsAcOEt) from Serjania schiedeana, select fractions (F-6, F-12, F-13, F-14), and one isolated compound, were evaluated in 12-O-tetradecanoylphorbol 13-acetate (TPA) ear edema and kaolin/carrageenan (KC)-induced monoarthritis assays. SsEtOAc induced edema inhibition of 90% (2.0 mg/ear), fractions showed activity within a range of 67-89%. Due to the fact F-14 showed the highest effect, it was separated, yielding a proanthocyanidin-type called epicatechin-(4β→ 8)-epicatechin-(4β→ 8, 2β→ O → 7) epicatechin (ETP). This compound (2.0 mg/ear) provoked 72% of edema inhibition (ED50 = 0.25 mg/ear, Emax = 52.9%). After 9 days of treatment, joint inflammation was decreasing, and on the last day, SsEtOAc (400 mg/kg), F-14 and ETP (10 mg/kg), SsEtOAc (200 mg/kg), methotrexate (MTX) 1.0 mg/kg and meloxicam (MEL) 1.5 mg/kg, produced an inhibition articulate edema of 94, 62, 36, 21, 80, and 54%, respectively. In the joint, pro-inflammatory molecules were elevated in animals without treatment (vehicle group, VEH). Treatments from S. schiedeana induced a decrease in the concentration of interleukin (IL)-1β, IL-17, and IL-6, and SsEtOAc at a higher dose diminished tumor necrosis factor (TNF-α). IL-10 and IL-4 were fewer in the VEH group in comparison with healthy mice; the animals with treatments from S. schiedeana induced an increment in the levels of these cytokines in joint and spleen.
- Subjects
ANTI-inflammatory agents; PROANTHOCYANIDINS; FLAVONOIDS; SERJANIA; ETHYL acetate; ARTHRITIS
- Publication
Molecules, 2017, Vol 22, Issue 6, p863
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules22060863