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- Title
Development and evaluation of a rapid recombinase polymerase amplification assay for detection of coxsackievirus A6.
- Authors
Wang, Kaifeng; Wu, Yue; Yin, Dan; Tang, Shixing; Hu, Guifang; He, Yaqing
- Abstract
Coxsackievirus A6 (CV-A6) is an important pathogen causing hand, foot and mouth disease (HFMD). The aim of this study was to develop and evaluate a rapid real-time reverse transcription recombinase polymerase amplification (RT-RPA) assay for detection of CV-A6. The sensitivity of this assay was 202 copies/reaction, with 100 % specificity. Furthermore, this assay yielded consistent results comparable with a commercial qRT-PCR diagnostic kit. This assay is therefore potentially useful for surveillance of CV-A6 infections and outbreak control.
- Subjects
HAND, foot &; mouth disease; COXSACKIEVIRUS diseases; RECOMBINASES; GENE amplification; POLYMERASE chain reaction; SENSITIVITY analysis; DIAGNOSIS; VIRUSES
- Publication
Archives of Virology, 2017, Vol 162, Issue 1, p287
- ISSN
0304-8608
- Publication type
Report
- DOI
10.1007/s00705-016-3100-8