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- Title
Lipofuscin Component A2E Does Not Reduce Antioxidant Activity of DOPA-Melanin.
- Authors
Dontsov, A.; Koromyslova, A.; Sakina, N.
- Abstract
We studied the capacity of DOPA-melanin (natural eumelanin analog) to bind chromophore A2E of retinal pigmented epithelial cell lipofuscin granule into complexes. DOPA-melanin bound up to 200 nm A2E per 1 mg polymer; antioxidant activity of the resultant complexes was evaluated. Luminol chemiluminescence quenching in the presence of hydrogen peroxide showed that the chemiluminescence latency/concentration constants were virtually the same for DOPA-melanin and its A2E complexes. Comparison of the inhibitory effects of DOPAmelanin and DOPA-A2E complexes by rate of UV-induced peroxidation of the outer segments of photoreceptor cells showed higher inhibitory activity of the complexes in comparison with pure DOPA-melanin. Antioxidant activity of DOPA-A2E complexes towards Fe-ascorbateinduced peroxidation of the outer segments of photoreceptor cells was also higher than that of DOPA-melanin. The results indicated that chromophore A2E of lipofuscin granules in the studied concentrations did not attenuate the antioxidant effects of DOPA-melanin and even potentiated it. This suggested that A2E excess in retinal pigmented epithelium cells could be bound by melanosome melanin and lose its toxicity.
- Subjects
DOPA; MELANINS; CHROMOPHORES; PHOTORECEPTORS; ANTIOXIDANTS; LIPOFUSCINS; PEROXIDATION
- Publication
Bulletin of Experimental Biology & Medicine, 2013, Vol 154, Issue 5, p624
- ISSN
0007-4888
- Publication type
Article
- DOI
10.1007/s10517-013-2015-6