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- Title
Inactivation of the Cytokinin Membrane Receptor AHK2 Gene Causes Differential Expression of Genes of Trans-Factors Involved in Regulation of Senescence in Arabidopsis thaliana.
- Authors
Kudryakova, N. V.; Danilova, M. N.; Andreeva, A. A.; Doroshenko, A. S.; Klepikova, A. V.; Shtratnikova, V. Yu.; Kusnetsov, V. V.
- Abstract
Senescence of leaves is initiated by differential expression of genes of transcription factors and is controlled by various phytohormones, among which cytokinins (CK), which negatively regulate this process, play an important role. According to the results of our transcriptome analysis and real-time PCR of selected genes, the delayed leaf senescence in mutants with an inactivated gene of CK receptor AHK2 was associated with altered expression of trans-factor genes belonging to the families AP2-EREBP, bHLH, C2H2, GATA, MYB, NAC, and WRKY. The most significant quantitative changes in the ahk2 mutants, as compared to the leaves of wild type plants, were observed in the expression profile of the bHLH38, bHLH39, and bHLH100 genes from the pool of iron uptake regulators. Significantly increased levels of transcripts of these genes were associated with the activation of the FEP2 (AT1G47395) and FEP3 (AT1G47400) genes, capable of inducing the expression of bHLH38 and bHLH39 under conditions of iron deficiency. The increased expression of the DREB26, ESE3, GATA4, ETC2, and F11O4.13 genes was accompanied by an increased accumulation of transcripts of the AGP17 gene of arabinogalactan protein as well as the IBL1, BEE2, Ole e 1, and GDSL genes associated with the regulation of cell wall proteins. Thus, the AHK2 receptor, usually is atributed with an auxiliary role in the perception of the CK signal by leaves, is associated with a specific set of genes involved in the regulation of iron outflow and degradation of cell wall components at the final stages of leaf ontogenesis.
- Subjects
ARABIDOPSIS thaliana; GENE expression; GENE families; GENES; GENETIC regulation
- Publication
Russian Journal of Plant Physiology, 2021, Vol 68, Issue 6, p1029
- ISSN
1021-4437
- Publication type
Article
- DOI
10.1134/S102144372106008X