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- Title
Bacterial nitrilases and their regulation.
- Authors
Chhiba-Govindjee, Varsha P.; van der Westhuyzen, Chris W.; Bode, Moira L.; Brady, Dean
- Abstract
Commercially, nitrilases are valuable biocatalysts capable of converting a diverse range of nitriles to carboxylic acids for the greener synthesis of chemicals and pharmaceuticals. Nitrilases are widespread in nature and are both important components of metabolic pathways and a response to environmental factors such as natural or manmade nitriles. Nitrilases are often grouped together on a genome in specific gene clusters that reflect these metabolic functions. Although nitrilase induction systems are still poorly understood, it is known that a powerful Rhodococcal transcription regulator system permits accumulation of intracellular nitrilase of up to 30–40% of total soluble protein in wild type Rhodococcous rhodochrous and host Streptomyces strains. Nitrilase expression inducer molecules encompass a broad range of aliphatic, aromatic and heteroaromatic nitriles, as well as some secondary and tertiary amides that are resistant to nitrilase degradation.
- Subjects
NITRILASES; NITRILES; CARBOXYLIC acids; GENE clusters; STREPTOMYCES
- Publication
Applied Microbiology & Biotechnology, 2019, Vol 103, Issue 12, p4679
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-019-09776-1