We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Protein engineering of microbial cholesterol oxidases: a molecular approach toward development of new enzymes with new properties.
- Authors
Moradpour, Zahra; Ghasemian, Abdollah
- Abstract
Cholesterol oxidase, a flavoenzyme, catalyzes two reactions in one active site: oxidation and isomerization. This enzyme has been isolated from a variety of microorganisms, mostly from actinomycetes. This enzyme has been widely used in clinical laboratories for cholesterol assays and was subsequently determined to have other potential applications. Engineering of cholesterol oxidase have enabled the identification of critical residues, and the information derived could lead to the rational development of improved types of the enzyme with increased stability and better functional properties. This review is the first that exclusively summarizes the reported results on the engineering of bacterial cholesterol oxidases aimed at improving their thermal and chemical stability, catalytic activity, and substrate specificity.
- Subjects
PROTEIN engineering; HYBRID enzymes; CATALYSTS; COFACTORS (Biochemistry); BIOCATALYSIS; ISOPENTENOIDS; MICROBIOLOGY; BIOTECHNOLOGY
- Publication
Applied Microbiology & Biotechnology, 2016, Vol 100, Issue 10, p4323
- ISSN
0175-7598
- Publication type
Article
- DOI
10.1007/s00253-016-7497-5