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- Title
Respiratory Syncytial Virus Utilizes a tRNA Fragment to Suppress Antiviral Responses Through a Novel Targeting Mechanism.
- Authors
Deng, Junfang; Ptashkin, Ryan N; Chen, Yu; Cheng, Zhi; Liu, Guangliang; Phan, Thien; Deng, Xiaoling; Zhou, Jiehua; Lee, Inhan; Lee, Yong Sun; Bao, Xiaoyong
- Abstract
Target identification is highly instructive in defining the biological roles of microRNAs. However, little is known about other small noncoding RNAs; for example, tRNA-derived RNA Fragments (tRFs). Some tRFs exhibit a gene-silencing mechanism distinctly different from that of typical microRNAs. We recently demonstrated that a respiratory syncytial virus (RSV)-induced tRF, called tRF5-GluCTC, promotes RSV replication. RSV is the single most important cause of lower respiratory tract infection in children. By using biochemical screening and bioinformatics analyses, we have identified apolipoprotein E receptor 2 (APOER2) as a target of tRF5-GluCTC. The 3′-portion of tRF5-GluCTC recognizes a target site in the 3′-untranslated region of APOER2 and suppresses its expression. We have also discovered that APOER2 is an anti-RSV protein whose suppression by tRF5-GluCTC promotes RSV replication. Our report represents the first identification of a natural target of a tRF and illustrates how a virus utilizes a host tRF to control a host gene to favor its replication.
- Subjects
RESPIRATORY syncytial virus genetics; TRANSFER RNA genetics; MICRORNA genetics; VIRAL genetics; BIOCHEMICAL genetics
- Publication
Molecular Therapy, 2015, Vol 23, Issue 10, p1622
- ISSN
1525-0016
- Publication type
Article
- DOI
10.1038/mt.2015.124