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- Title
HPLC ASSAY OF CAFFEINE AND PARAXANTHINE FOR CYP1A2 PHENOTYPE ASSESSMENT.
- Authors
REHMAN, S.; RAZZAQ, A.; KAMAL, S.; BIBI, I.; KIRAN, S.; NOUREN, S.; AYUB, C. M.; HAMID, M.
- Abstract
Cytochrome P4501A2 (CYP1A2), as a drug metabolising enzyme, has a great influence on the metabolism of many commonly used medicines with potential involvement in the bio-activation and detoxification of carcinogens. Therefore, in vivo measurement of CYP1A2 activity using a probe drug can be an important tool to investigate some critical factors that influence variability in drug pharmacokinetics. Caffeine is the only, in practice, accepted probe drug to measure the CYP1A2 phenotyping characteristics with caffeine clearance or the paraxanthine to caffeine ratio as the preferred metric. The purpose of present study was to determine the caffeine and its metabolite phenotypes in relation to cancer risk in healthy male volunteers of local population in Pakistan. The average value of metabolic ratio [(1,7-dimethylxanthine (17X) and caffeine 1,3,7-trimethylxanthine (137X)] was found to be 1.023 ± 0.31. In the present study, a Shim Pack CLC-ODS (150×4.6 mm, 5 m pore size) high-performance liquid chromatography (HPLC) column was used to quantify caffeine and paraxanthine (major metabolites of caffeine) concentrations in plasma. Retention time was 12.91 and 14.866 min for 17X and 137X, respectively. The linearity of calibration curve of 137X and 17X were covered 0 to 12 µg/ml (R2 = 0.994). A significant positive correlation was observed between metabolic ratio and cancer risk factors. All of the volunteers were found as fast metabolisers, consequently, CYP1A2 activity may relate to cancer incidence or high risk of cancerous diseases.
- Subjects
CAFFEINE; XANTHINE; HIGH performance liquid chromatography; CYTOCHROME P-450; CANCER; PHARMACOKINETICS
- Publication
Oxidation Communications, 2016, Vol 39, Issue 3-I, p2344
- ISSN
0209-4541
- Publication type
Article